Hotspot

rbcL-atpB

LSC · 25543-26330 · 788 bp

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Pi 0.00457

MarkerSeek score 54.70

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 6
pi: 0.00457
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	ACTCCGGGGTGTAGTAAGTC	TATCCAGCACGGGTCCAATA	55.0	50.0	58.2	58.2	970-974 bp	1.0	77.9
rbcL-atpB_p2	CTCCGGGGTGTAGTAAGTCA	TATCCAGCACGGGTCCAATA	55.0	50.0	58.2	58.2	969-973 bp	1.0	77.9
rbcL-atpB_p3	ACTCCGGGGTGTAGTAAGTC	CCAGCACGGGTCCAATAATT	55.0	50.0	58.2	58.2	967-971 bp	1.0	77.6
rbcL-atpB_p4	CTCCGGGGTGTAGTAAGTCA	CCAGCACGGGTCCAATAATT	55.0	50.0	58.2	58.2	966-970 bp	1.0	77.6
rbcL-atpB_p5	ACTCCGGGGTGTAGTAAGTC	CCGGGGGAAAAGTGATATCC	55.0	55.0	58.2	57.7	985-989 bp	1.0	77.3