Hotspot

psbJ-petA

LSC · 19047-19936 · 890 bp

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Pi 0.00517

MarkerSeek score 55.80

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: LSC
length_bp: 890
variable_sites: 10
pi: 0.00517
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	TGGTTCGTATTCTGGATTGGG	GGCCTTTTGTTCTTCTTCGC	47.6	50.0	57.7	57.9	1025-1032 bp	1.0	70.6
psbJ-petA_p2	GGTTCGTATTCTGGATTGGGT	GGCCTTTTGTTCTTCTTCGC	47.6	50.0	57.7	57.9	1024-1031 bp	1.0	70.6
psbJ-petA_p3	TGGTTCGTATTCTGGATTGGG	CGTCCAAGGCCTTTTGTTCT	47.6	50.0	57.7	58.7	1032-1039 bp	1.0	65.6
psbJ-petA_p4	GGTTCGTATTCTGGATTGGGT	CGTCCAAGGCCTTTTGTTCT	47.6	50.0	57.7	58.7	1031-1038 bp	1.0	65.6
psbJ-petA_p5	TGGTTCGTATTCTGGATTGGG	GCCTTTTGTTCTTCTTCGCA	47.6	45.0	57.7	57.2	1024-1031 bp	1.0	64.8