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petL-psbE

LSC · 16996-18277 · 1282 bp

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Pi 0.0064

MarkerSeek score 52.90

Primer available yes

Label petL-psbE

Feature Metadata

label_name: petL-psbE
feature_type: igs
region: LSC
length_bp: 1282
variable_sites: 15
pi: 0.0064
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-psbE_p1	AAGCAGCCAGTAGAAAACCG	TGACAAATAACCAACCCGCA	50.0	45.0	58.5	58.0	1440-1469 bp	1.0	77.5
petL-psbE_p2	GCAGCCAGTAGAAAACCGAA	TGACAAATAACCAACCCGCA	50.0	45.0	58.5	58.0	1438-1467 bp	1.0	77.5
petL-psbE_p3	AAGCAGCCAGTAGAAAACCG	CAACCCGCAATGAATAGGGA	50.0	50.0	58.5	57.9	1429-1458 bp	1.0	77.3
petL-psbE_p4	GCAGCCAGTAGAAAACCGAA	CAACCCGCAATGAATAGGGA	50.0	50.0	58.5	57.9	1427-1456 bp	1.0	77.2
petL-psbE_p5	AAGCAGCCAGTAGAAAACCG	ACCAACCCGCAATGAATAGG	50.0	50.0	58.5	58.2	1431-1460 bp	1.0	75.8