Hotspot

rps8-infA

LSC · 3862-4033 · 172 bp

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Pi 0.05

MarkerSeek score 83.30

Primer available yes

Label rps8-infA

Feature Metadata

label_name: rps8-infA
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 15
pi: 0.05
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-infA_p1	ATTCCTAAGGTTTTGGGCGG	TCGTGTCATTCTCTAGGCGA	50.0	50.0	58.2	58.3	305-323 bp	1.0	84.6
rps8-infA_p2	CCTAAGGTTTTGGGCGGAAT	TCGTGTCATTCTCTAGGCGA	50.0	50.0	58.2	58.3	302-320 bp	1.0	84.6
rps8-infA_p3	ATTCCTAAGGTTTTGGGCGG	TTGACCCTATCCCCCATCAG	50.0	55.0	58.2	58.2	375-393 bp	1.0	84.2
rps8-infA_p4	CCTAAGGTTTTGGGCGGAAT	TTGACCCTATCCCCCATCAG	50.0	55.0	58.2	58.2	372-390 bp	1.0	84.2
rps8-infA_p5	CCTAAGGTTTTGGGCGGAAT	ATAGCCCAGGATGATCGTGT	50.0	50.0	58.2	58.3	316-334 bp	1.0	84.2