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rpl22-rps3

LSC · 447-509 · 63 bp

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Pi 0.0286

MarkerSeek score 59.40

Primer available yes

Label rpl22-rps3

Feature Metadata

label_name: rpl22-rps3
feature_type: igs
region: LSC
length_bp: 63
variable_sites: 4
pi: 0.0286
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps3_p1	CGAGCTCGAGGACGTAGTTA	GTGCGAACCAAAAGGAATGG	55.0	50.0	58.7	57.9	220-239 bp	1.0	75.5
rpl22-rps3_p2	CGAGCTCGAGGACGTAGTTA	ACCAAGTCTGAAACCGAGTG	55.0	50.0	58.7	57.8	186-205 bp	1.0	74.4
rpl22-rps3_p3	CGAGCTCGAGGACGTAGTTA	CCAAGTCTGAAACCGAGTGG	55.0	55.0	58.7	58.5	185-204 bp	1.0	72.5
rpl22-rps3_p4	CGAGCTCGAGGACGTAGTTA	CAAGTCTGAAACCGAGTGGA	55.0	50.0	58.7	57.5	184-203 bp	1.0	72.2
rpl22-rps3_p5	CGAGCTCGAGGACGTAGTTA	GTTGTGCGAACCAAAAGGAA	55.0	45.0	58.7	57.4	223-242 bp	1.0	71.5