Hotspot

ycf1

SSC · 125054-130663 · 5610 bp

Back to Spiraea

Pi 0.00763

MarkerSeek score 46.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5610
variable_sites: 218
pi: 0.00763
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGGTTTATTTTTGAGAGTTCT	TGGAATGGAAGCTGAACTACC	31.8	47.6	53.5	57.6	241 bp	0.96875	45.1
ycf1_p2	TGGGTTTATTTTTGAGAGTTCTT	TGGAATGGAAGCTGAACTACC	30.4	47.6	54.4	57.6	241 bp	0.96875	45.1
ycf1_p3	TGGGTTTATTTTTGAGAGTTCT	GGAATGGAAGCTGAACTACCT	31.8	47.6	53.5	57.4	240 bp	0.96875	45.1
ycf1_p4	TGGGTTTATTTTTGAGAGTTCTTT	TGGAATGGAAGCTGAACTACC	29.2	47.6	55.1	57.6	241 bp	0.96875	45.1
ycf1_p5	TTGGGTTTATTTTTGAGAGTTCT	TGGAATGGAAGCTGAACTACC	30.4	47.6	54.4	57.6	242 bp	0.96875	45.0