Hotspot

ndhF-rpl32

SSC · 112928-113992 · 1065 bp

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Pi 0.0126

MarkerSeek score 59.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1065
variable_sites: 66
pi: 0.0126
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCCACATACGACGAAGATT	TTCAATGCTGCCCGATATCC	50.0	50.0	58.1	58.1	1177-1298 bp	1.0	87.0
ndhF-rpl32_p2	CCCCAACGCGATTAGTTACA	TTCAATGCTGCCCGATATCC	50.0	50.0	58.0	58.1	1611-1732 bp	1.0	86.6
ndhF-rpl32_p3	ACAAACGCTTTTTGACAGGC	TTCAATGCTGCCCGATATCC	45.0	50.0	58.1	58.1	1594-1715 bp	1.0	86.1
ndhF-rpl32_p4	CAAACGCTTTTTGACAGGCA	TTCAATGCTGCCCGATATCC	45.0	50.0	58.1	58.1	1593-1714 bp	1.0	86.1
ndhF-rpl32_p5	CCCCAACGCGATTAGTTACA	AAAAGCTTTCAATGCTGCCC	50.0	45.0	58.0	57.8	1618-1739 bp	1.0	86.0