Hotspot

psbE-petL

LSC · 65445-66746 · 1302 bp

Back to Spiraea

Pi 0.00551

MarkerSeek score 53.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1302
variable_sites: 44
pi: 0.00551
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.8	58.2	1405-1442 bp	0.96875	81.5
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCG	55.0	55.0	58.2	58.2	1400-1437 bp	0.96875	81.2
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	TCGTATCTTGCTCAGACCGA	50.0	50.0	57.8	58.3	1404-1441 bp	0.96875	80.8
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	TCGTATCTTGCTCAGACCGA	55.0	50.0	58.2	58.3	1399-1436 bp	0.96875	80.5
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GTCGTATCTTGCTCAGACCG	50.0	55.0	58.4	58.2	1398-1435 bp	0.96875	80.0