Hotspot

ycf4-cemA

LSC · 61750-62089 · 340 bp

Back to Spiraea

Pi 0.0125

MarkerSeek score 59.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 340
variable_sites: 25
pi: 0.0125
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGAAAGAGATACACCAAGGCA	45.0	45.5	58.1	57.8	454-700 bp	1.0	68.2
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	TGAGAAAGAGATACACCAAGGC	45.0	45.5	58.1	57.8	455-701 bp	1.0	68.2
ycf4-cemA_p3	AAAAAGCTGCTGAATTGGCC	GAGAAAGAGATACACCAAGGCA	45.0	45.5	57.8	57.8	468-714 bp	1.0	67.4
ycf4-cemA_p4	AAAAAGCTGCTGAATTGGCC	TGAGAAAGAGATACACCAAGGC	45.0	45.5	57.8	57.8	469-715 bp	1.0	67.4
ycf4-cemA_p5	AAAGCTGCTGAATTGGCCTA	GAGAAAGAGATACACCAAGGCA	45.0	45.5	57.8	57.8	466-712 bp	1.0	67.1