Hotspot

ndhC-trnV-UAC

LSC · 52134-52639 · 506 bp

Back to Spiraea

Pi 0.0123

MarkerSeek score 56.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 506
variable_sites: 25
pi: 0.0123
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	600-701 bp	1.0	84.5
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	664-765 bp	1.0	84.4
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	AATGCACGTTGGGTCTTTGA	50.0	45.0	57.9	58.3	667-768 bp	1.0	83.2
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	ATGCACGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	666-767 bp	1.0	83.2
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCGGAAAT	AAATGCACGTTGGGTCTTTG	50.0	45.0	57.9	57.5	668-769 bp	1.0	81.7