Hotspot

psbM-trnD-GUC

LSC · 30233-31421 · 1189 bp

Back to Spiraea

Pi 0.00714
MarkerSeek score 57.20
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1189
variable_sites
41
pi
0.00714
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TTAATCGCCTTGGCTGACTG TTCAATTGGTCAGAGCACCG 50.0 50.0 58.3 58.5 1173-1380 bp 1.0 80.6
psbM-trnD-GUC_p2 ATTAATCGCCTTGGCTGACT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.3 58.5 1174-1381 bp 1.0 77.0
psbM-trnD-GUC_p3 TTAATCGCCTTGGCTGACTG GTTCAATTGGTCAGAGCACC 50.0 50.0 58.3 57.0 1174-1381 bp 1.0 76.4
psbM-trnD-GUC_p4 AATTAATCGCCTTGGCTGACT TTCAATTGGTCAGAGCACCG 42.9 50.0 58.0 58.5 1175-1382 bp 1.0 74.7
psbM-trnD-GUC_p5 GCCTTGGCTGACTGTTTTTAC TTCAATTGGTCAGAGCACCG 47.6 50.0 58.0 58.5 1167-1374 bp 1.0 74.6