Hotspot

rpoB-trnC-GCA

LSC · 27370-28439 · 1070 bp

Back to Spiraea

Pi 0.00807

MarkerSeek score 60.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1070
variable_sites: 39
pi: 0.00807
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTGCTTCATTTCCACCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1146-1181 bp	1.0	80.3
rpoB-trnC-GCA_p2	CTCTGTTAAGCCCTGCTCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1219-1254 bp	1.0	79.6
rpoB-trnC-GCA_p3	ATTGCTTCATTTCCACCCCC	TCTTTTGGTGATCAGGCGAC	50.0	50.0	58.4	58.2	1168-1203 bp	1.0	79.4
rpoB-trnC-GCA_p4	GTTCCTCTGTTAAGCCCTGC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.5	58.1	1223-1258 bp	1.0	79.0
rpoB-trnC-GCA_p5	CTCTGTTAAGCCCTGCTCAA	TCTTTTGGTGATCAGGCGAC	50.0	50.0	57.5	58.2	1241-1276 bp	1.0	78.7