Hotspot

trnR-UCU-atpA

LSC · 10073-10597 · 525 bp

Back to Spiraea

Pi 0.013

MarkerSeek score 50.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 525
variable_sites: 34
pi: 0.013
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAATGAAAAGCGTCCATTG	ACGTTTTCTACTTCAGGAACA	42.9	38.1	57.4	55.1	510-649 bp	1.0	48.5
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	ACGTTTTCTACTTCAGGAACA	42.9	38.1	57.4	55.1	509-648 bp	1.0	48.5
trnR-UCU-atpA_p3	GGAATGAAAAGCGTCCATTGT	GAACGTTTTCTACTTCAGGAACA	42.9	39.1	57.4	57.0	511-650 bp	1.0	48.5
trnR-UCU-atpA_p4	TGGAATGAAAAGCGTCCATTG	CGTTTTCTACTTCAGGAACAAGT	42.9	39.1	57.4	57.0	509-648 bp	1.0	48.5
trnR-UCU-atpA_p5	TGGAATGAAAAGCGTCCATTG	GAACGTTTTCTACTTCAGGAACA	42.9	39.1	57.4	57.0	512-651 bp	1.0	48.4