Hotspot

rpoC2

LSC · 42523-46608 · 4086 bp

Back to Sphenostylis

Pi 0.00245

MarkerSeek score 40.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4086
variable_sites: 10
pi: 0.00245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TCCCAGGGAGTTCACATTCT	AAGTGACCGGAGATGAATGC	50.0	50.0	58.0	58.0	573 bp	1.0	81.4
rpoC2_p2	GGCTAACCCTTATTTGGCGA	AAGTGACCGGAGATGAATGC	50.0	50.0	58.0	58.0	895 bp	1.0	80.6
rpoC2_p3	AGGGCGTTCCTTAGAAGAGT	GTGCTACTGAATCAGCTGCT	50.0	50.0	58.1	58.0	923 bp	1.0	80.6
rpoC2_p4	TCCCAGGGAGTTCACATTCT	GTGCTACTGAATCAGCTGCT	50.0	50.0	58.0	58.0	1063 bp	1.0	80.5
rpoC2_p5	GGCTAACCCTTATTTGGCGA	GTGCTACTGAATCAGCTGCT	50.0	50.0	58.0	58.0	1385 bp	1.0	80.3