Hotspot

rpl16

LSC · 813-2231 · 1419 bp

Back to Sphenostylis

Pi 0.00352

MarkerSeek score 46.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1419
variable_sites: 5
pi: 0.00352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATTGCGCGTGTAGAATGGAT	GTCTCAATTCTCGGGCGATT	45.0	50.0	58.0	58.1	2168-2171 bp	1.0	79.4
rpl16_p2	ATTGCGCGTGTAGAATGGAT	TCTCAATTCTCGGGCGATTG	45.0	50.0	58.0	58.1	2167-2170 bp	1.0	79.4
rpl16_p3	ATTGCGCGTGTAGAATGGAT	AACTGTCTCAATTCTCGGGC	45.0	50.0	58.0	57.9	2172-2175 bp	1.0	79.1
rpl16_p4	ATTGCGCGTGTAGAATGGAT	ACCTCGGGTGCTAATGAAAC	45.0	50.0	58.0	57.9	2208-2211 bp	1.0	79.0
rpl16_p5	ATTGCGCGTGTAGAATGGAT	GCGCAAAAGATTCGAGTTCC	45.0	50.0	58.0	58.1	2148-2151 bp	1.0	79.0