Hotspot

clpP

LSC · 68211-70314 · 2104 bp

Back to Sphenostylis

Pi 0.00385

MarkerSeek score 46.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2104
variable_sites: 8
pi: 0.00385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CACATACACCCTGGTACACG	AACAGGGTCAGAGGGATCAA	55.0	50.0	58.0	58.0	2962-2985 bp	1.0	80.7
clpP_p2	CACATACACCCTGGTACACG	ACAGGGTCAGAGGGATCAAA	55.0	50.0	58.0	58.0	2961-2984 bp	1.0	80.7
clpP_p3	CTGAACGAGTCGCACATACA	AACAGGGTCAGAGGGATCAA	50.0	50.0	58.0	58.0	2974-2997 bp	1.0	80.5
clpP_p4	CTGAACGAGTCGCACATACA	ACAGGGTCAGAGGGATCAAA	50.0	50.0	58.0	58.0	2973-2996 bp	1.0	80.5
clpP_p5	GTGACGTTTCGAATTGGCTG	ACAGGGTCAGAGGGATCAAA	50.0	50.0	58.0	58.0	2900-2923 bp	1.0	80.3