Hotspot

ndhI-ndhG

SSC · 115271-115548 · 278 bp

Back to Sphenostylis

Pi 0.0144

MarkerSeek score 61.10

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 278
variable_sites: 4
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	TCCTTTTATGATCGCCACGA	AGCGATTCGAACAAATCCAT	45.0	40.0	57.0	55.8	402-403 bp	1.0	55.8
ndhI-ndhG_p2	TCCTTTTATGATCGCCACGA	GTAGCGATTCGAACAAATCCA	45.0	42.9	57.0	56.7	404-405 bp	1.0	55.0
ndhI-ndhG_p3	TCCTTTTATGATCGCCACGA	TGTAGCGATTCGAACAAATCC	45.0	42.9	57.0	56.7	405-406 bp	1.0	55.0
ndhI-ndhG_p4	TCCTTTTATGATCGCCACGA	TGTAGCGATTCGAACAAATCCA	45.0	40.9	57.0	58.4	405-406 bp	1.0	53.9
ndhI-ndhG_p5	TCCTTTTATGATCGCCACGAA	AGCGATTCGAACAAATCCAT	42.9	40.0	57.7	55.8	402-403 bp	1.0	53.6