Hotspot

psaJ-rps18

LSC · 65298-66162 · 865 bp

Back to Sphenostylis

Pi 0.00462

MarkerSeek score 61.40

Primer available yes

Label psaJ-rps18

Feature Metadata

label_name: psaJ-rps18
feature_type: igs
region: LSC
length_bp: 865
variable_sites: 4
pi: 0.00462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rps18_p1	CATATCTTTCCGTGGCACCA	CGGAGGCAAACGTCTATGAA	50.0	50.0	58.0	58.0	1047-1057 bp	1.0	80.0
psaJ-rps18_p2	ACATATCTTTCCGTGGCACC	CGGAGGCAAACGTCTATGAA	50.0	50.0	58.0	58.0	1048-1058 bp	1.0	80.0
psaJ-rps18_p3	GGCTTTGGCAGGTCTATTGA	CGGAGGCAAACGTCTATGAA	50.0	50.0	57.9	58.0	1004-1014 bp	1.0	79.3
psaJ-rps18_p4	CATATCTTTCCGTGGCACCA	ATTGAATCGGAGGCAAACGT	50.0	45.0	58.0	58.2	1054-1064 bp	1.0	78.6
psaJ-rps18_p5	ACATATCTTTCCGTGGCACC	ATTGAATCGGAGGCAAACGT	50.0	45.0	58.0	58.2	1055-1065 bp	1.0	78.6