Hotspot

ycf4-cemA

LSC · 59592-60093 · 502 bp

Back to Sphenostylis

Pi 0.00794

MarkerSeek score 60.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 502
variable_sites: 3
pi: 0.00794
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGCAAATAAAGCTGGCGAA	ACACCAGGGCAAAAAGACTA	40.0	45.0	56.9	57.0	488-624 bp	1.0	67.3
ycf4-cemA_p2	TTGCAAATAAAGCTGGCGAA	TACACCAGGGCAAAAAGACT	40.0	45.0	56.9	57.0	489-625 bp	1.0	67.3
ycf4-cemA_p3	TTGCAAATAAAGCTGGCGAA	GAAAGAGATACACCAGGGCA	40.0	50.0	56.9	56.9	497-633 bp	1.0	67.0
ycf4-cemA_p4	TTGCAAATAAAGCTGGCGAA	TGAAAGAGATACACCAGGGC	40.0	50.0	56.9	56.9	498-634 bp	1.0	67.0
ycf4-cemA_p5	GGTGTACCGCTTCTGTATTGA	ACACCAGGGCAAAAAGACTA	47.6	45.0	57.8	57.0	453-589 bp	1.0	66.2