Hotspot

trnD-GUC-psbM

LSC · 33456-33842 · 387 bp

Back to Sphenostylis

Pi 0.00785

MarkerSeek score 60.10

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 387
variable_sites: 3
pi: 0.00785
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	AGGAACTAGAATAAACAGTGCAGT	50.0	37.5	58.5	57.9	501-505 bp	1.0	44.5
trnD-GUC-psbM_p2	TACTGTACACCCCTTTCCCT	AGGAACTAGAATAAACAGTGCAGT	50.0	37.5	57.3	57.9	531-535 bp	1.0	42.5
trnD-GUC-psbM_p3	TTCAATTGGTCAGAGCACCG	GGAACTAGAATAAACAGTGCAGT	50.0	39.1	58.5	56.5	500-504 bp	1.0	41.8
trnD-GUC-psbM_p4	TTCAATTGGTCAGAGCACCG	ACTAGAATAAACAGTGCAGTAGC	50.0	39.1	58.5	56.4	497-501 bp	1.0	41.3
trnD-GUC-psbM_p5	ACTGTACACCCCTTTCCCTG	AGGAACTAGAATAAACAGTGCAGT	55.0	37.5	58.9	57.9	530-534 bp	1.0	41.2