Hotspot

trnT-GGU-trnE-UUC

LSC · 32096-32823 · 728 bp

Back to Sphenostylis

Pi 0.00277
MarkerSeek score 61.00
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
728
variable_sites
2
pi
0.00277
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 CCATGGCGTTACTCTACCAC GCTGCCTCCTTGAAAGAGAG 55.0 55.0 58.1 58.3 805-810 bp 1.0 78.1
trnT-GGU-trnE-UUC_p2 CCATGGCGTTACTCTACCAC TGTCCTGAACCACTAGACGA 55.0 50.0 58.1 57.7 784-789 bp 1.0 77.9
trnT-GGU-trnE-UUC_p3 TGGCGTTACTCTACCACTGA GCTGCCTCCTTGAAAGAGAG 50.0 55.0 58.1 58.3 802-807 bp 1.0 77.9
trnT-GGU-trnE-UUC_p4 ACACCTTACCATGGCGTTAC GCTGCCTCCTTGAAAGAGAG 50.0 55.0 57.9 58.3 813-818 bp 1.0 77.8
trnT-GGU-trnE-UUC_p5 TGGCGTTACTCTACCACTGA TGTCCTGAACCACTAGACGA 50.0 50.0 58.1 57.7 781-786 bp 1.0 77.8