Pi
0.00254
MarkerSeek score
60.30
Primer available
yes
Label
trnL-UAA-trnT-UGU
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnL-UAA-trnT-UGU_p1 | ACCAATTTCGCCATATCCCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.0 | 57.9 | 839-840 bp | 1.0 | 79.2 |
| trnL-UAA-trnT-UGU_p2 | TAGCGTCTACCAATTTCGCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.1 | 57.9 | 847-848 bp | 1.0 | 78.7 |
| trnL-UAA-trnT-UGU_p3 | ACCAATTTCGCCATATCCCC | GGATTCGAACCGATGACCAT | 50.0 | 50.0 | 58.0 | 57.8 | 871-872 bp | 1.0 | 78.3 |
| trnL-UAA-trnT-UGU_p4 | TAGCGTCTACCAATTTCGCC | GGATTCGAACCGATGACCAT | 50.0 | 50.0 | 58.1 | 57.8 | 879-880 bp | 1.0 | 77.8 |
| trnL-UAA-trnT-UGU_p5 | CCCATTGTGAATTCCAGGGT | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 57.8 | 57.9 | 932-933 bp | 1.0 | 77.3 |