Hotspot

ndhJ-trnF-GAA

LSC · 14970-15763 · 794 bp

Back to Sphenostylis

Pi 0.00252
MarkerSeek score 60.20
Primer available yes
Label ndhJ-trnF-GAA

Feature Metadata

label_name
ndhJ-trnF-GAA
feature_type
igs
region
LSC
length_bp
794
variable_sites
2
pi
0.00252
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-trnF-GAA_p1 ATAGTCATCCGCGTCTGAGA GCTTCAAGAATGGTCGGGAT 50.0 50.0 58.0 58.0 989 bp 1.0 79.6
ndhJ-trnF-GAA_p2 ATAGTCATCCGCGTCTGAGA CCTCGTGTCACCAGTTCAAA 50.0 50.0 58.0 58.1 938 bp 1.0 79.5
ndhJ-trnF-GAA_p3 TTGGATAGGATGGCCTTTGC GCTTCAAGAATGGTCGGGAT 50.0 50.0 57.9 58.0 949 bp 1.0 79.2
ndhJ-trnF-GAA_p4 ATAGTCATCCGCGTCTGAGA AGAATGGTCGGGATAGCTCA 50.0 50.0 58.0 57.9 983 bp 1.0 79.2
ndhJ-trnF-GAA_p5 TTGGATAGGATGGCCTTTGC CCTCGTGTCACCAGTTCAAA 50.0 50.0 57.9 58.1 898 bp 1.0 79.2