Hotspot

petN

Genome · 90432-90560 · 129 bp

Back to Sphagnum

Pi 0.0446

MarkerSeek score 67.30

Primer available yes

Label petN

Feature Metadata

label_name: petN
feature_type: gene
region: Genome
length_bp: 129
variable_sites: 13
pi: 0.0446
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN_p1	TCCCCATCCCGATCAAGTAA	CCATTCATTAAGGCACCCCA	50.0	50.0	57.8	57.8	605-609 bp	0.4	55.6
petN_p2	GCCAATCGATACTCTCCACC	CCATTCATTAAGGCACCCCA	55.0	50.0	57.8	57.8	949-954 bp	0.4	55.5
petN_p3	GCCAATCGATACTCTCCACC	ACCATTCATTAAGGCACCCC	55.0	50.0	57.8	57.8	950-955 bp	0.4	55.5
petN_p4	GCCAAGTGGATTAAGGCAGA	CCATTCATTAAGGCACCCCA	50.0	50.0	57.9	57.8	450-459 bp	0.4	54.9
petN_p5	GCCAAGTGGATTAAGGCAGA	ACCATTCATTAAGGCACCCC	50.0	50.0	57.9	57.8	451-460 bp	0.4	54.9