Hotspot

rrn23-trnA-UGC

Genome · 133642-133887 · 246 bp

Back to Sphagnum

Pi 0.0244

MarkerSeek score 71.00

Primer available yes

Label rrn23-trnA-UGC

Feature Metadata

label_name: rrn23-trnA-UGC
feature_type: igs
region: Genome
length_bp: 246
variable_sites: 14
pi: 0.0244
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23-trnA-UGC_p1	ATTCGGGAATCTCCGGATCT	GAGAGGCCTGAAAGATGGTG	50.0	55.0	58.0	58.0	352-438 bp	1.0	86.3
rrn23-trnA-UGC_p2	ATTCGGGAATCTCCGGATCT	AGAGAGGCCTGAAAGATGGT	50.0	50.0	58.0	58.0	353-439 bp	1.0	86.3
rrn23-trnA-UGC_p3	TATTTTCAGCTCCCCGAAGC	GAGAGGCCTGAAAGATGGTG	50.0	55.0	58.0	58.0	327-413 bp	1.0	86.3
rrn23-trnA-UGC_p4	TATTTTCAGCTCCCCGAAGC	AGAGAGGCCTGAAAGATGGT	50.0	50.0	58.0	58.0	328-414 bp	1.0	86.3
rrn23-trnA-UGC_p5	GCTTATTTTCAGCTCCCCGA	GAGAGGCCTGAAAGATGGTG	50.0	55.0	58.0	58.0	330-416 bp	1.0	86.2