Hotspot

petN-ycf66

Genome · 90561-90796 · 236 bp

Back to Sphagnum

Pi 0.0426

MarkerSeek score 68.10

Primer available yes

Label petN-ycf66

Feature Metadata

label_name: petN-ycf66
feature_type: igs
region: Genome
length_bp: 236
variable_sites: 27
pi: 0.0426
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-ycf66_p1	TACTGGAAAATTCGCGGGAG	CACTTCGGGAACATATGCGA	50.0	50.0	58.0	58.1	1058-1073 bp	1.0	86.1
petN-ycf66_p2	TACTGGAAAATTCGCGGGAG	TCACTTCGGGAACATATGCG	50.0	50.0	58.0	58.1	1059-1074 bp	1.0	86.1
petN-ycf66_p3	TACTGGAAAATTCGCGGGAG	ATCCACGGGGTGTAGAATGA	50.0	50.0	58.0	58.1	925-940 bp	1.0	86.1
petN-ycf66_p4	TGGAAAATTCGCGGGAGAAA	CACTTCGGGAACATATGCGA	45.0	50.0	58.1	58.1	1055-1070 bp	1.0	85.5
petN-ycf66_p5	TGGAAAATTCGCGGGAGAAA	TCACTTCGGGAACATATGCG	45.0	50.0	58.1	58.1	1056-1071 bp	1.0	85.5