Hotspot

rpoC1-rpoB

Genome · 86620-86645 · 26 bp

Back to Sphagnum

Pi 0.0291

MarkerSeek score 63.50

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: Genome
length_bp: 26
variable_sites: 2
pi: 0.0291
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	CAACTACGGATCTGTTCGGG	TTACGATCCCTAGCTCTGGG	55.0	55.0	58.1	58.0	171-173 bp	1.0	80.7
rpoC1-rpoB_p2	CCAACTACGGATCTGTTCGG	TTACGATCCCTAGCTCTGGG	55.0	55.0	58.1	58.0	172-174 bp	1.0	80.7
rpoC1-rpoB_p3	CCCAACTACGGATCTGTTCG	TTACGATCCCTAGCTCTGGG	55.0	55.0	58.1	58.0	173-175 bp	1.0	80.7
rpoC1-rpoB_p4	AACTACGGATCTGTTCGGGA	TTACGATCCCTAGCTCTGGG	50.0	55.0	58.2	58.0	170-172 bp	1.0	80.1
rpoC1-rpoB_p5	AGTTCTTTCGGCCCAACTAC	TTACGATCCCTAGCTCTGGG	50.0	55.0	57.8	58.0	184-186 bp	1.0	79.8