Hotspot

trnD-GUC-ycf2

Genome · 54766-55447 · 682 bp

Back to Sphagnum

Pi 0.0168

MarkerSeek score 65.90

Primer available yes

Label trnD-GUC-ycf2

Feature Metadata

label_name: trnD-GUC-ycf2
feature_type: igs
region: Genome
length_bp: 682
variable_sites: 33
pi: 0.0168
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-ycf2_p1	TTAGAGTACCGCCCTGTCAA	AAAAATGAGATTGGACACTTCA	50.0	31.8	58.1	54.2	745-779 bp	1.0	47.7
trnD-GUC-ycf2_p2	TTAGAGTACCGCCCTGTCAA	AAATGAGATTGGACACTTCA	50.0	35.0	58.1	52.4	743-777 bp	1.0	47.6
trnD-GUC-ycf2_p3	TTAGAGTACCGCCCTGTCAA	AAAATGAGATTGGACACTTCA	50.0	33.3	58.1	53.3	744-778 bp	1.0	47.6
trnD-GUC-ycf2_p4	TTAGAGTACCGCCCTGTCAA	AATGAGATTGGACACTTCAT	50.0	35.0	58.1	52.1	742-776 bp	1.0	47.6
trnD-GUC-ycf2_p5	TTAGAGTACCGCCCTGTCAA	ATGAGATTGGACACTTCATT	50.0	35.0	58.1	52.1	741-775 bp	1.0	47.6