Hotspot

psbD-trnT-GGU

Genome · 52289-53281 · 993 bp

Back to Sphagnum

Pi 0.0136

MarkerSeek score 65.80

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 993
variable_sites: 42
pi: 0.0136
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TAGGCCAGACCAACCTACAA	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.0	58.1	1145-1186 bp	1.0	85.0
psbD-trnT-GGU_p2	TAGGCCAGACCAACCTACAA	TACTCGATTACTCGCCCCTT	50.0	50.0	58.0	57.9	1172-1213 bp	1.0	84.9
psbD-trnT-GGU_p3	TAGGCCAGACCAACCTACAA	TTACTCGATTACTCGCCCCT	50.0	50.0	58.0	57.9	1173-1214 bp	1.0	84.9
psbD-trnT-GGU_p4	TAGGCCAGACCAACCTACAA	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.0	58.1	1148-1189 bp	1.0	84.8
psbD-trnT-GGU_p5	ACGGTCTCTTCTTAGCCAGT	TACTCGATTACTCGCCCCTT	50.0	50.0	58.1	57.9	1145-1186 bp	1.0	84.7