Hotspot

ndhF

SSC · 108426-110645 · 2220 bp

Back to Sphagneticola

Pi 0.0149

MarkerSeek score 51.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 33
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCCGAGAAGAAGGTATCAGC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	58.1	58.0	2788-2842 bp	1.0	72.9
ndhF_p2	CTAGCACTGGGTAGACCTCA	GAGGTAAGATCCGGTGAATCG	55.0	52.4	57.9	58.0	2704-2758 bp	1.0	72.9
ndhF_p3	TCGTTGTGGTCGGACTCTAT	GAGGTAAGATCCGGTGAATCG	50.0	52.4	58.2	58.0	2892-2946 bp	1.0	72.6
ndhF_p4	ATCAATTCGGTCGTTGTGGT	GAGGTAAGATCCGGTGAATCG	45.0	52.4	57.8	58.0	2902-2956 bp	1.0	72.5
ndhF_p5	AGAAGAAGGAGCCGAGAAGA	GAGGTAAGATCCGGTGAATCG	50.0	52.4	57.8	58.0	2798-2852 bp	1.0	72.2