Hotspot

ndhG-ndhI

SSC · 117194-117566 · 373 bp

Back to Sphagneticola

Pi 0.0321
MarkerSeek score 64.30
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
373
variable_sites
11
pi
0.0321
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 TCATGTATTAGTCCAGGTAAATCCA TCAAATTGCTTTGGGTCGGT 36.0 45.0 57.3 58.3 463-487 bp 1.0 45.9
ndhG-ndhI_p2 TCATGTATTAGTCCAGGTAAATCCA CAAATTGCTTTGGGTCGGTT 36.0 45.0 57.3 57.5 462-486 bp 1.0 45.9
ndhG-ndhI_p3 CATGTATTAGTCCAGGTAAATCCA TCAAATTGCTTTGGGTCGGT 37.5 45.0 56.0 58.3 462-486 bp 1.0 45.9
ndhG-ndhI_p4 TGTATTAGTCCAGGTAAATCCA TCAAATTGCTTTGGGTCGGT 36.4 45.0 54.0 58.3 460-484 bp 1.0 45.9
ndhG-ndhI_p5 CATGTATTAGTCCAGGTAAATCCA CAAATTGCTTTGGGTCGGTT 37.5 45.0 56.0 57.5 461-485 bp 1.0 45.9