Hotspot

psaC-ndhE

SSC · 115900-116144 · 245 bp

Back to Sphagneticola

Pi 0.0252

MarkerSeek score 67.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 245
variable_sites: 6
pi: 0.0252
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	CTGAAGCCGCTATTGGACTC	50.0	55.0	58.1	58.4	436-439 bp	1.0	78.0
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	CCGCTATTGGACTCGCTATT	50.0	50.0	58.1	57.6	430-433 bp	1.0	77.9
psaC-ndhE_p3	TTTGGCTTTACATCCGTCCC	CTGAAGCCGCTATTGGACTC	50.0	55.0	58.2	58.4	445-448 bp	1.0	77.6
psaC-ndhE_p4	TTTGGCTTTACATCCGTCCC	CCGCTATTGGACTCGCTATT	50.0	50.0	58.2	57.6	439-442 bp	1.0	77.5
psaC-ndhE_p5	GGACACATTGAGTACACCCG	CTGAAGCCGCTATTGGACTC	55.0	55.0	58.3	58.4	389-392 bp	1.0	77.1