Hotspot

ndhF-rpl32

SSC · 110646-111652 · 1007 bp

Back to Sphagneticola

Pi 0.0357
MarkerSeek score 72.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1007
variable_sites
35
pi
0.0357
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG TCCAAATATTTTTACGAATACGCT 50.0 29.2 58.0 55.3 1150-1166 bp 1.0 44.3
ndhF-rpl32_p2 TGCCGTCGGAAAAAGAAGAA TCCAAATATTTTTACGAATACGCT 45.0 29.2 58.0 55.3 1148-1164 bp 1.0 44.3
ndhF-rpl32_p3 TTGCCGTCGGAAAAAGAAGA TCCAAATATTTTTACGAATACGCT 45.0 29.2 58.0 55.3 1149-1165 bp 1.0 44.3
ndhF-rpl32_p4 CTTTTTGTTGCCGTCGGAAA TCCAAATATTTTTACGAATACGCT 45.0 29.2 57.8 55.3 1156-1172 bp 1.0 44.3
ndhF-rpl32_p5 TTTGTTGCCGTCGGAAAAAG TCCAAATATTTTTACGAATACGCT 45.0 29.2 57.8 55.3 1153-1169 bp 1.0 44.3