Hotspot

ycf1-ndhF

SSC · 108341-108425 · 85 bp

Back to Sphagneticola

Pi 0.278
MarkerSeek score 77.10
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
85
variable_sites
22
pi
0.278
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 CTAGCACTGGGTAGACCTCA GGGGGTGGACGTATTTCTTC 55.0 55.0 57.9 58.0 517-565 bp 1.0 84.9
ycf1-ndhF_p2 CTAGCACTGGGTAGACCTCA TGTGGGGGTGAGAAAATTGG 55.0 50.0 57.9 58.0 635-683 bp 1.0 84.5
ycf1-ndhF_p3 GCCGAGAAGAAGGTATCAGC GGGGGTGGACGTATTTCTTC 55.0 55.0 58.1 58.0 601-649 bp 1.0 84.1
ycf1-ndhF_p4 GCCGAGAAGAAGGTATCAGC TGTGGGGGTGAGAAAATTGG 55.0 50.0 58.1 58.0 719-767 bp 1.0 83.9
ycf1-ndhF_p5 TCGTTGTGGTCGGACTCTAT TGTGGGGGTGAGAAAATTGG 50.0 50.0 58.2 58.0 823-871 bp 1.0 83.0