Hotspot

ndhC-trnV-UAC

LSC · 49656-50415 · 760 bp

Back to Sphagneticola

Pi 0.0506

MarkerSeek score 91.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 37
pi: 0.0506
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 91.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTTGCTACTCGGAGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	876-904 bp	1.0	83.2
ndhC-trnV-UAC_p2	ACTCGGAGCTAAAAATCCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	867-895 bp	1.0	81.9
ndhC-trnV-UAC_p3	TTCTCCGGTCCTTTGCTACT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	884-912 bp	1.0	81.4
ndhC-trnV-UAC_p4	TTTCTCCGGTCCTTTGCTAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	885-913 bp	1.0	80.6
ndhC-trnV-UAC_p5	TCCTTTGCTACTCGGAGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	875-903 bp	1.0	80.5