Hotspot

psaA-D3Q48_pgp065

LSC · 41373-42098 · 726 bp

Back to Sphagneticola

Pi 0.0277
MarkerSeek score 65.70
Primer available yes
Label psaA-D3Q48_pgp065

Feature Metadata

label_name
psaA-D3Q48_pgp065
feature_type
igs
region
LSC
length_bp
726
variable_sites
20
pi
0.0277
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-D3Q48_pgp065_p1 GAGAAATGACCCGGTCTAGC GCTATAGCACTTACTCCTGGT 55.0 47.6 57.8 56.6 891-896 bp 1.0 61.3
psaA-D3Q48_pgp065_p2 GAGAAATGACCCGGTCTAGC TGGTTGAAGATCACTAGACGT 55.0 42.9 57.8 56.4 849-854 bp 1.0 59.9
psaA-D3Q48_pgp065_p3 TTTTACTTCTGGTTCCGGCG GCTATAGCACTTACTCCTGGT 50.0 47.6 58.5 56.6 826-831 bp 1.0 59.5
psaA-D3Q48_pgp065_p4 TTTTACTTCTGGTTCCGGCG TGGTTGAAGATCACTAGACGT 50.0 42.9 58.5 56.4 784-789 bp 1.0 58.0
psaA-D3Q48_pgp065_p5 GAGAAATGACCCGGTCTAGC TGGTTGAAGATCACTAGACGTT 55.0 40.9 57.8 57.1 849-854 bp 1.0 57.6