Hotspot

trnS-GCU-trnC-GCA

LSC · 8636-9385 · 750 bp

Back to Sphagneticola

Pi 0.0214

MarkerSeek score 63.10

Primer available yes

Label trnS-GCU-trnC-GCA

Feature Metadata

label_name: trnS-GCU-trnC-GCA
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 16
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	901-902 bp	1.0	79.6
trnS-GCU-trnC-GCA_p2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	883-884 bp	1.0	77.5
trnS-GCU-trnC-GCA_p3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.6	58.1	837-838 bp	1.0	75.9
trnS-GCU-trnC-GCA_p4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	902-903 bp	1.0	75.5
trnS-GCU-trnC-GCA_p5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.6	58.4	819-820 bp	1.0	73.7