Hotspot

ccsA-ndhD

Genome · 115599-116029 · 431 bp

Back to Sphaeropteris

Pi 0.0116

MarkerSeek score 62.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: Genome
length_bp: 431
variable_sites: 5
pi: 0.0116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGATTCGTTTTTCGGGAGT	TGGTTCTACATCTATGGAAAAGT	45.0	34.8	57.4	55.0	562-563 bp	1.0	40.9
ccsA-ndhD_p2	GCAGTGGCATCTACAGGATT	TGTCTCTTTCTACCAATACTAGGT	50.0	37.5	57.7	56.3	635-636 bp	1.0	40.8
ccsA-ndhD_p3	TGGATTCGTTTTTCGGGAGT	TGTCTCTTTCTACCAATACTAGGT	45.0	37.5	57.4	56.3	605-606 bp	1.0	40.8
ccsA-ndhD_p4	GCAGTGGCATCTACAGGATT	TGGTTCTACATCTATGGAAAAGT	50.0	34.8	57.7	55.0	592-593 bp	1.0	40.8
ccsA-ndhD_p5	GCAGTGGCATCTACAGGATT	TTGTCTCTTTCTACCAATACTAGGT	50.0	36.0	57.7	57.0	636-637 bp	1.0	40.8