Hotspot

trnL-UAG-ccsA

Genome · 114354-114632 · 279 bp

Back to Sphaeropteris

Pi 0.0328

MarkerSeek score 64.30

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: Genome
length_bp: 279
variable_sites: 9
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	CCTAGATCGATCCAATGCGG	50.0	55.0	58.0	58.0	431-436 bp	1.0	82.5
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	CCTAGATCGATCCAATGCGG	50.0	55.0	58.0	58.0	430-435 bp	1.0	82.5
trnL-UAG-ccsA_p3	GTGAAATCGGTAGACACGCT	CAATGCGGCAAAGTAACAGG	50.0	50.0	58.0	58.0	419-424 bp	1.0	82.4
trnL-UAG-ccsA_p4	GTGAAATCGGTAGACACGCT	CCAATGCGGCAAAGTAACAG	50.0	50.0	58.0	58.0	420-425 bp	1.0	82.4
trnL-UAG-ccsA_p5	TGAAATCGGTAGACACGCTG	CAATGCGGCAAAGTAACAGG	50.0	50.0	58.0	58.0	418-423 bp	1.0	82.4