Hotspot

rpl32-trnP-GGG

Genome · 113402-114025 · 624 bp

Back to Sphaeropteris

Pi 0.0259
MarkerSeek score 73.50
Primer available yes
Label rpl32-trnP-GGG

Feature Metadata

label_name
rpl32-trnP-GGG
feature_type
igs
region
Genome
length_bp
624
variable_sites
16
pi
0.0259
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnP-GGG_p1 GGGCTAAAGCGGTGAAAGTA TCGTATGGGGGTAACGGAAT 50.0 50.0 57.9 58.2 828 bp 0.5 57.5
rpl32-trnP-GGG_p2 GGGCTAAAGCGGTGAAAGTA GGGGTAACGGAATATAGCGC 50.0 55.0 57.9 58.3 821 bp 0.5 56.9
rpl32-trnP-GGG_p3 GGGCTAAAGCGGTGAAAGTA TACTCGTATGGGGGTAACGG 50.0 55.0 57.9 58.3 831 bp 0.5 56.7
rpl32-trnP-GGG_p4 GGGCTAAAGCGGTGAAAGTA GGGGGTAACGGAATATAGCG 50.0 55.0 57.9 57.6 822 bp 0.5 56.2
rpl32-trnP-GGG_p5 GGGCTAAAGCGGTGAAAGTA GGGTAACGGAATATAGCGCA 50.0 50.0 57.9 57.6 820 bp 0.5 55.6