Hotspot

trnT-UGU-trnR-ACG

Genome · 88001-89098 · 1098 bp

Back to Sphaeropteris

Pi 0.00924

MarkerSeek score 65.50

Primer available yes

Label trnT-UGU-trnR-ACG

Feature Metadata

label_name: trnT-UGU-trnR-ACG
feature_type: igs
region: Genome
length_bp: 1098
variable_sites: 10
pi: 0.00924
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnR-ACG_p1	ACGTTCTCCTGCCTCGATAT	AAAAGTGGTTCCTGGAACGG	50.0	50.0	58.3	58.3	1293-1309 bp	1.0	76.6
trnT-UGU-trnR-ACG_p2	TACGTTCTCCTGCCTCGATA	AAAAGTGGTTCCTGGAACGG	50.0	50.0	57.7	58.3	1294-1310 bp	1.0	76.4
trnT-UGU-trnR-ACG_p3	TACTACGTTCTCCTGCCTCG	AAAAGTGGTTCCTGGAACGG	55.0	50.0	58.6	58.3	1297-1313 bp	1.0	74.1
trnT-UGU-trnR-ACG_p4	TCTACTACGTTCTCCTGCCT	AAAAGTGGTTCCTGGAACGG	50.0	50.0	57.2	58.3	1299-1315 bp	1.0	72.7
trnT-UGU-trnR-ACG_p5	ACGTTCTCCTGCCTCGATAT	AAGTGGTTCCTGGAACGGAT	50.0	50.0	58.3	58.9	1291-1307 bp	1.0	71.7