Hotspot

infA-rps8

Genome · 79463-79599 · 137 bp

Back to Sphaeropteris

Pi 0.0219

MarkerSeek score 65.10

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: Genome
length_bp: 137
variable_sites: 3
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	AACATGGCATTGGGAAGTGA	GGGGGAAATCCTATGCCATG	45.0	55.0	57.7	58.1	233 bp	1.0	78.0
infA-rps8_p2	AACATGGCATTGGGAAGTGA	CTTGTGACAGATCGAGAGGC	45.0	55.0	57.7	58.1	271 bp	1.0	77.8
infA-rps8_p3	AACATGGCATTGGGAAGTGA	CGGACTTGTGACAGATCGAG	45.0	55.0	57.7	58.1	275 bp	1.0	77.6
infA-rps8_p4	GAGTTGAGACTTGGCATCCA	GGGGGAAATCCTATGCCATG	50.0	55.0	57.5	58.1	271 bp	1.0	76.5
infA-rps8_p5	TGAGTTGAGACTTGGCATCC	GGGGGAAATCCTATGCCATG	50.0	55.0	57.5	58.1	272 bp	1.0	76.5