Hotspot

rpl20-rps12

Genome · 67691-68745 · 1055 bp

Back to Sphaeropteris

Pi 0.0105
MarkerSeek score 65.40
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
Genome
length_bp
1055
variable_sites
11
pi
0.0105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CCCCTTAAATCCGGATGCAA CGAGGAGTATGTACCAGGGT 50.0 55.0 57.9 57.9 1150-1157 bp 1.0 80.2
rpl20-rps12_p2 CCCCTTAAATCCGGATGCAA CGATTGGGGAGTGGAACAAA 50.0 50.0 57.9 58.1 1201-1208 bp 1.0 79.9
rpl20-rps12_p3 CCCCTTAAATCCGGATGCAA CAACGGCGAGGAGTATGTAC 50.0 55.0 57.9 58.2 1156-1163 bp 1.0 79.4
rpl20-rps12_p4 CCCCTTAAATCCGGATGCAA AAGACAACGATTGGGGAGTG 50.0 50.0 57.9 57.8 1208-1215 bp 1.0 79.2
rpl20-rps12_p5 CCCCTTAAATCCGGATGCAA CAAGACAACGATTGGGGAGT 50.0 50.0 57.9 57.8 1209-1216 bp 1.0 79.2