Hotspot

trnS-GCU-psaM

Genome · 8078-8419 · 342 bp

Back to Sphaeropteris

Pi 0.0323

MarkerSeek score 72.20

Primer available yes

Label trnS-GCU-psaM

Feature Metadata

label_name: trnS-GCU-psaM
feature_type: igs
region: Genome
length_bp: 342
variable_sites: 11
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psaM_p1	ATTAGCAATCCGACGCGTTA	GTTGCGAGATTGGCTATTGC	45.0	50.0	57.8	57.9	415-424 bp	1.0	79.7
trnS-GCU-psaM_p2	TTAGACCTCTCGGCCATCTC	GTTGCGAGATTGGCTATTGC	55.0	50.0	58.3	57.9	398-407 bp	1.0	79.0
trnS-GCU-psaM_p3	TCTTGGAGGAGAGAGAGGGA	GTTGCGAGATTGGCTATTGC	55.0	50.0	58.3	57.9	469-478 bp	1.0	78.6
trnS-GCU-psaM_p4	ATTAGCAATCCGACGCGTTA	TTGCGAGATTGGCTATTGCA	45.0	45.0	57.8	58.3	414-423 bp	1.0	78.6
trnS-GCU-psaM_p5	ATTAGCAATCCGACGCGTTA	GGCTATTGCACTGTACCGTT	45.0	50.0	57.8	58.3	404-413 bp	1.0	78.5