primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	matK-rps16	1	TTTCCGTAACGTCCCAAACC	GGGCAAAGGTACCTGAACAA	20	20	50.0	50.0	58.4	58.0	0.0	17.1	0.418230	3046	4026	1064-1067	1.000000	77.8
matK-rps16_p2	matK-rps16	2	ACTTTTCCGTAACGTCCCAA	GGGCAAAGGTACCTGAACAA	20	20	45.0	50.0	57.4	58.0	0.0	17.1	0.628735	3046	4026	1067-1070	1.000000	76.1
matK-rps16_p3	matK-rps16	3	TTTCCGTAACGTCCCAAACC	ATATCGAAACGGGCAAAGGT	20	20	50.0	45.0	58.4	57.6	0.0	0.0	0.821246	3046	4026	1074-1077	1.000000	74.5
matK-rps16_p4	matK-rps16	4	TTTCCGTAACGTCCCAAACC	GGGGCTCAAACAACAGAAAC	20	20	50.0	50.0	58.4	57.5	0.0	0.0	0.916989	3046	4026	1104-1107	1.000000	73.7
matK-rps16_p5	matK-rps16	5	TTTCCGTAACGTCCCAAACC	ATCGAAACGGGCAAAGGTAC	20	20	50.0	50.0	58.4	58.6	0.0	0.0	0.962066	3046	4026	1072-1075	1.000000	73.4
trnS-GCU-psaM_p1	trnS-GCU-psaM	1	ATTAGCAATCCGACGCGTTA	GTTGCGAGATTGGCTATTGC	20	20	45.0	50.0	57.8	57.9	15.5	0.0	0.323909	8078	8419	415-424	1.000000	79.7
trnS-GCU-psaM_p2	trnS-GCU-psaM	2	TTAGACCTCTCGGCCATCTC	GTTGCGAGATTGGCTATTGC	20	20	55.0	50.0	58.3	57.9	2.2	0.0	0.417414	8078	8419	398-407	1.000000	79.0
trnS-GCU-psaM_p3	trnS-GCU-psaM	3	TCTTGGAGGAGAGAGAGGGA	GTTGCGAGATTGGCTATTGC	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.423548	8078	8419	469-478	1.000000	78.6
trnS-GCU-psaM_p4	trnS-GCU-psaM	4	ATTAGCAATCCGACGCGTTA	TTGCGAGATTGGCTATTGCA	20	20	45.0	45.0	57.8	58.3	15.5	0.7	0.463918	8078	8419	414-423	1.000000	78.6
trnS-GCU-psaM_p5	trnS-GCU-psaM	5	ATTAGCAATCCGACGCGTTA	GGCTATTGCACTGTACCGTT	20	20	45.0	50.0	57.8	58.3	15.5	0.7	0.478706	8078	8419	404-413	1.000000	78.5
ycf66_p1	ycf66	1	TTCGTAGCCACTGCACTTTT	CTGCAGTTTGGGGAAGAAGT	20	20	45.0	50.0	58.0	58.0	0.0	5.2	0.051324	29120	29822	1303-1307	1.000000	81.4
ycf66_p2	ycf66	2	AGGTTGCAACAGCTGATTGA	CTGCAGTTTGGGGAAGAAGT	20	20	45.0	50.0	58.0	58.0	29.6	5.2	0.027644	29120	29822	1608-1612	1.000000	81.2
ycf66_p3	ycf66	3	TGAGGTTGCAACAGCTGATT	CTGCAGTTTGGGGAAGAAGT	20	20	45.0	50.0	58.0	58.0	29.6	5.2	0.027644	29120	29822	1610-1614	1.000000	81.2
ycf66_p4	ycf66	4	AGGTTGCAACAGCTGATTGA	AGACGGAGGATTGCAAATCC	20	20	45.0	50.0	58.0	58.0	29.6	1.7	0.058650	29120	29822	2122-2124	1.000000	80.8
ycf66_p5	ycf66	5	TGAGGTTGCAACAGCTGATT	AGACGGAGGATTGCAAATCC	20	20	45.0	50.0	58.0	58.0	29.6	1.7	0.058650	29120	29822	2124-2126	1.000000	80.8
trnfM-CAU-rps14_p1	trnfM-CAU-rps14	1	AAGGTTATGAGCCTTGCGAG	CGACCCAGATCCAATTACCG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.163973	38261	38402	287-308	1.000000	80.0
trnfM-CAU-rps14_p2	trnfM-CAU-rps14	2	TTTGAACCCGTGACCTCAAG	CGACCCAGATCCAATTACCG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.172342	38261	38402	304-325	1.000000	79.9
trnfM-CAU-rps14_p3	trnfM-CAU-rps14	3	ACAGGATTTGAACCCGTGAC	CGACCCAGATCCAATTACCG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.241926	38261	38402	310-331	1.000000	79.3
trnfM-CAU-rps14_p4	trnfM-CAU-rps14	4	GATTTGAACCCGTGACCTCA	CGACCCAGATCCAATTACCG	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.313047	38261	38402	306-327	1.000000	78.8
trnfM-CAU-rps14_p5	trnfM-CAU-rps14	5	CCTTTGCGGAGACAGGATTT	CGACCCAGATCCAATTACCG	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.307750	38261	38402	321-342	1.000000	78.7
rps4-trnL-CAA_p1	rps4-trnL-CAA	1	TCCCTGTAAGCCCTGGTAAA	CTGAATCTGGGAGCTGTCAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.069125	47327	47980	812-813	1.000000	80.1
rps4-trnL-CAA_p2	rps4-trnL-CAA	2	TTCCCTGTAAGCCCTGGTAA	CTGAATCTGGGAGCTGTCAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.069125	47327	47980	813-814	1.000000	80.1
rps4-trnL-CAA_p3	rps4-trnL-CAA	3	TTTCCCTGTAAGCCCTGGTA	CTGAATCTGGGAGCTGTCAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.069125	47327	47980	814-815	1.000000	80.1
rps4-trnL-CAA_p4	rps4-trnL-CAA	4	AAGCAGATTGATCAGCAGCA	CTGAATCTGGGAGCTGTCAC	20	20	45.0	55.0	57.9	58.0	14.4	0.0	0.145632	47327	47980	860-861	1.000000	79.5
rps4-trnL-CAA_p5	rps4-trnL-CAA	5	AGCAGATTGATCAGCAGCAA	CTGAATCTGGGAGCTGTCAC	20	20	45.0	55.0	57.9	58.0	14.4	0.0	0.145632	47327	47980	859-860	1.000000	79.5
accD-psaI_p1	accD-psaI	1	GAAGGGTGTTTTGAGCGAGA	AGGGTAGATAGGACGCTGTC	20	20	50.0	55.0	58.1	58.0	0.0	0.8	0.136981	57849	58413	648-656	1.000000	79.4
accD-psaI_p2	accD-psaI	2	GAAGGGTGTTTTGAGCGAGA	GAGGGTAGATAGGACGCTGT	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.136981	57849	58413	649-657	1.000000	79.4
accD-psaI_p3	accD-psaI	3	AGCCTTATTACTCAGCCCCT	AGGGTAGATAGGACGCTGTC	20	20	50.0	55.0	57.8	58.0	0.0	0.8	0.214268	57849	58413	622-630	1.000000	78.8
accD-psaI_p4	accD-psaI	4	AGCCTTATTACTCAGCCCCT	GAGGGTAGATAGGACGCTGT	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.214268	57849	58413	623-631	1.000000	78.8
accD-psaI_p5	accD-psaI	5	TGAGCGAGATATCTGAGCCT	AGGGTAGATAGGACGCTGTC	20	20	50.0	55.0	57.7	58.0	20.2	0.8	0.300688	57849	58413	637-645	1.000000	78.1
petA-psbJ_p1	petA-psbJ	1	TGGCACAGATCTTTCTCGTG	ATAGGCTCGCTGGGGATATT	20	20	50.0	50.0	57.9	58.0	5.9	0.0	0.126321	62376	63128	884-913	1.000000	80.2
petA-psbJ_p2	petA-psbJ	2	TCCAGTTGGCGGAAATGAAT	ATAGGCTCGCTGGGGATATT	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.256088	62376	63128	839-868	1.000000	79.3
petA-psbJ_p3	petA-psbJ	3	GCACAGATCTTTCTCGTGCT	ATAGGCTCGCTGGGGATATT	20	20	50.0	50.0	58.3	58.0	21.6	0.0	0.322112	62376	63128	882-911	1.000000	78.7
petA-psbJ_p4	petA-psbJ	4	CCGTTGCGTATTCAAGGTCT	ATAGGCTCGCTGGGGATATT	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.319700	62376	63128	930-959	1.000000	78.6
petA-psbJ_p5	petA-psbJ	5	TGGCACAGATCTTTCTCGTG	TAGGCTCGCTGGGGATATTT	20	20	50.0	50.0	57.9	58.3	5.9	0.0	0.355023	62376	63128	883-912	1.000000	78.4
rpl20-rps12_p1	rpl20-rps12	1	CCCCTTAAATCCGGATGCAA	CGAGGAGTATGTACCAGGGT	20	20	50.0	55.0	57.9	57.9	19.9	0.0	0.134733	67691	68745	1150-1157	1.000000	80.2
rpl20-rps12_p2	rpl20-rps12	2	CCCCTTAAATCCGGATGCAA	CGATTGGGGAGTGGAACAAA	20	20	50.0	50.0	57.9	58.1	19.9	0.0	0.161612	67691	68745	1201-1208	1.000000	79.9
rpl20-rps12_p3	rpl20-rps12	3	CCCCTTAAATCCGGATGCAA	CAACGGCGAGGAGTATGTAC	20	20	50.0	55.0	57.9	58.2	19.9	16.6	0.229515	67691	68745	1156-1163	1.000000	79.4
rpl20-rps12_p4	rpl20-rps12	4	CCCCTTAAATCCGGATGCAA	AAGACAACGATTGGGGAGTG	20	20	50.0	50.0	57.9	57.8	19.9	0.0	0.250903	67691	68745	1208-1215	1.000000	79.2
rpl20-rps12_p5	rpl20-rps12	5	CCCCTTAAATCCGGATGCAA	CAAGACAACGATTGGGGAGT	20	20	50.0	50.0	57.9	57.8	19.9	0.0	0.250903	67691	68745	1209-1216	1.000000	79.2
infA-rps8_p1	infA-rps8	1	AACATGGCATTGGGAAGTGA	GGGGGAAATCCTATGCCATG	20	20	45.0	55.0	57.7	58.1	0.0	0.0	0.378628	79463	79599	233-233	1.000000	78.0
infA-rps8_p2	infA-rps8	2	AACATGGCATTGGGAAGTGA	CTTGTGACAGATCGAGAGGC	20	20	45.0	55.0	57.7	58.1	0.0	0.0	0.387578	79463	79599	271-271	1.000000	77.8
infA-rps8_p3	infA-rps8	3	AACATGGCATTGGGAAGTGA	CGGACTTGTGACAGATCGAG	20	20	45.0	55.0	57.7	58.1	0.0	0.0	0.405958	79463	79599	275-275	1.000000	77.6
infA-rps8_p4	infA-rps8	4	GAGTTGAGACTTGGCATCCA	GGGGGAAATCCTATGCCATG	20	20	50.0	55.0	57.5	58.1	0.0	0.0	0.550698	79463	79599	271-271	1.000000	76.5
infA-rps8_p5	infA-rps8	5	TGAGTTGAGACTTGGCATCC	GGGGGAAATCCTATGCCATG	20	20	50.0	55.0	57.5	58.1	0.0	0.0	0.550698	79463	79599	272-272	1.000000	76.5
trnT-UGU-trnR-ACG_p1	trnT-UGU-trnR-ACG	1	ACGTTCTCCTGCCTCGATAT	AAAAGTGGTTCCTGGAACGG	20	20	50.0	50.0	58.3	58.3	0.0	28.1	0.618811	88001	89098	1293-1309	1.000000	76.6
trnT-UGU-trnR-ACG_p2	trnT-UGU-trnR-ACG	2	TACGTTCTCCTGCCTCGATA	AAAAGTGGTTCCTGGAACGG	20	20	50.0	50.0	57.7	58.3	0.0	28.1	0.649382	88001	89098	1294-1310	1.000000	76.4
trnT-UGU-trnR-ACG_p3	trnT-UGU-trnR-ACG	3	TACTACGTTCTCCTGCCTCG	AAAAGTGGTTCCTGGAACGG	20	20	55.0	50.0	58.6	58.3	0.0	28.1	0.935894	88001	89098	1297-1313	1.000000	74.1
trnT-UGU-trnR-ACG_p4	trnT-UGU-trnR-ACG	4	TCTACTACGTTCTCCTGCCT	AAAAGTGGTTCCTGGAACGG	20	20	50.0	50.0	57.2	58.3	0.0	28.1	1.115467	88001	89098	1299-1315	1.000000	72.7
trnT-UGU-trnR-ACG_p5	trnT-UGU-trnR-ACG	5	ACGTTCTCCTGCCTCGATAT	AAGTGGTTCCTGGAACGGAT	20	20	50.0	50.0	58.3	58.9	0.0	28.1	1.241549	88001	89098	1291-1307	1.000000	71.7
ndhF-rpl21_p1	ndhF-rpl21	1	GCTACTAGTGGGCACAAAGG	GGTTCCACTCGGAGTTGTTT	20	20	55.0	50.0	58.3	58.0	5.4	8.2	0.297761	112448	112669	334-337	1.000000	78.6
ndhF-rpl21_p2	ndhF-rpl21	2	ACTAGTGGGCACAAAGGAAC	GGTTCCACTCGGAGTTGTTT	20	20	50.0	50.0	57.7	58.0	0.0	8.2	0.308772	112448	112669	331-334	1.000000	78.5
ndhF-rpl21_p3	ndhF-rpl21	3	GCTACTAGTGGGCACAAAGG	TGTTTCCCACCAACGTCAAT	20	20	55.0	45.0	58.3	57.9	5.4	0.0	0.320984	112448	112669	319-322	1.000000	78.4
ndhF-rpl21_p4	ndhF-rpl21	4	ACTAGTGGGCACAAAGGAAC	TGTTTCCCACCAACGTCAAT	20	20	50.0	45.0	57.7	57.9	0.0	0.0	0.331994	112448	112669	316-319	1.000000	78.4
ndhF-rpl21_p5	ndhF-rpl21	5	GCGAAGACCTTTCGTAGCTT	GGTTCCACTCGGAGTTGTTT	20	20	50.0	50.0	58.3	58.0	10.5	8.2	0.325622	112448	112669	392-395	1.000000	78.2
rpl32-trnP-GGG_p1	rpl32-trnP-GGG	1	GGGCTAAAGCGGTGAAAGTA	TCGTATGGGGGTAACGGAAT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.311422	113402	114025	828-828	0.500000	57.5
rpl32-trnP-GGG_p2	rpl32-trnP-GGG	2	GGGCTAAAGCGGTGAAAGTA	GGGGTAACGGAATATAGCGC	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.385141	113402	114025	821-821	0.500000	56.9
rpl32-trnP-GGG_p3	rpl32-trnP-GGG	3	GGGCTAAAGCGGTGAAAGTA	TACTCGTATGGGGGTAACGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.412341	113402	114025	831-831	0.500000	56.7
rpl32-trnP-GGG_p4	rpl32-trnP-GGG	4	GGGCTAAAGCGGTGAAAGTA	GGGGGTAACGGAATATAGCG	20	20	50.0	55.0	57.9	57.6	0.0	0.0	0.478710	113402	114025	822-822	0.500000	56.2
rpl32-trnP-GGG_p5	rpl32-trnP-GGG	5	GGGCTAAAGCGGTGAAAGTA	GGGTAACGGAATATAGCGCA	20	20	50.0	50.0	57.9	57.6	0.0	11.5	0.547424	113402	114025	820-820	0.500000	55.6
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	GTGAAATCGGTAGACACGCT	CCTAGATCGATCCAATGCGG	20	20	50.0	55.0	58.0	58.0	0.0	21.7	0.009642	114354	114632	431-436	1.000000	82.5
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TGAAATCGGTAGACACGCTG	CCTAGATCGATCCAATGCGG	20	20	50.0	55.0	58.0	58.0	0.0	21.7	0.011013	114354	114632	430-435	1.000000	82.5
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	GTGAAATCGGTAGACACGCT	CAATGCGGCAAAGTAACAGG	20	20	50.0	50.0	58.0	58.0	0.0	6.8	0.026577	114354	114632	419-424	1.000000	82.4
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	GTGAAATCGGTAGACACGCT	CCAATGCGGCAAAGTAACAG	20	20	50.0	50.0	58.0	58.0	0.0	6.8	0.026577	114354	114632	420-425	1.000000	82.4
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	TGAAATCGGTAGACACGCTG	CAATGCGGCAAAGTAACAGG	20	20	50.0	50.0	58.0	58.0	0.0	6.8	0.027947	114354	114632	418-423	1.000000	82.4
ccsA-ndhD_p1	ccsA-ndhD	1	TGGATTCGTTTTTCGGGAGT	TGGTTCTACATCTATGGAAAAGT	20	23	45.0	34.8	57.4	55.0	0.0	0.0	6.506059	115599	116029	562-563	1.000000	40.9
ccsA-ndhD_p2	ccsA-ndhD	2	GCAGTGGCATCTACAGGATT	TGTCTCTTTCTACCAATACTAGGT	20	24	50.0	37.5	57.7	56.3	0.0	0.0	6.020701	115599	116029	635-636	1.000000	40.8
ccsA-ndhD_p3	ccsA-ndhD	3	TGGATTCGTTTTTCGGGAGT	TGTCTCTTTCTACCAATACTAGGT	20	24	45.0	37.5	57.4	56.3	0.0	0.0	6.236495	115599	116029	605-606	1.000000	40.8
ccsA-ndhD_p4	ccsA-ndhD	4	GCAGTGGCATCTACAGGATT	TGGTTCTACATCTATGGAAAAGT	20	23	50.0	34.8	57.7	55.0	0.0	0.0	6.290266	115599	116029	592-593	1.000000	40.8
ccsA-ndhD_p5	ccsA-ndhD	5	GCAGTGGCATCTACAGGATT	TTGTCTCTTTCTACCAATACTAGGT	20	25	50.0	36.0	57.7	57.0	0.0	0.0	6.370226	115599	116029	636-637	1.000000	40.8
ndhH-rps15_p1	ndhH-rps15	1	GGAGAACACCATGCATCGAT	CGCAAGCGTCTCTCAATCTA	20	20	50.0	50.0	58.0	57.8	0.0	6.2	0.235758	123355	123465	286-286	1.000000	79.0
ndhH-rps15_p2	ndhH-rps15	2	GGAGAACACCATGCATCGAT	ATTCGAGGATTAAAGGGGCG	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.297076	123355	123465	214-214	1.000000	78.7
ndhH-rps15_p3	ndhH-rps15	3	CAATAAGCCGGAGAACACCA	CGCAAGCGTCTCTCAATCTA	20	20	50.0	50.0	57.9	57.8	5.0	6.2	0.302750	123355	123465	295-295	1.000000	78.4
ndhH-rps15_p4	ndhH-rps15	4	ACAATAAGCCGGAGAACACC	CGCAAGCGTCTCTCAATCTA	20	20	50.0	50.0	57.9	57.8	5.0	6.2	0.304224	123355	123465	296-296	1.000000	78.4
ndhH-rps15_p5	ndhH-rps15	5	CAATAAGCCGGAGAACACCA	ATTCGAGGATTAAAGGGGCG	20	20	50.0	50.0	57.9	57.7	5.0	0.0	0.364067	123355	123465	223-223	1.000000	78.2
rps15-ycf1_p1	rps15-ycf1	1	AGCAGATCCTGTGCCTTTTT	CAGCCACCGTCTAGAAGAAC	20	20	45.0	55.0	57.7	58.0	2.0	7.3	0.301657	123736	124869	1308-1332	1.000000	79.0
rps15-ycf1_p2	rps15-ycf1	2	AGCAGATCCTGTGCCTTTTT	AGCCACCGTCTAGAAGAACT	20	20	45.0	50.0	57.7	58.1	2.0	7.3	0.373218	123736	124869	1307-1331	1.000000	78.4
rps15-ycf1_p3	rps15-ycf1	3	AGCAGATCCTGTGCCTTTTT	TTGATTCGCCATGCTCAAGA	20	20	45.0	45.0	57.7	57.9	2.0	0.0	0.411706	123736	124869	1245-1269	1.000000	78.2
rps15-ycf1_p4	rps15-ycf1	4	CAGCAGATCCTGTGCCTTTT	CAGCCACCGTCTAGAAGAAC	20	20	50.0	55.0	58.5	58.0	2.0	7.3	0.459838	123736	124869	1309-1333	1.000000	77.7
rps15-ycf1_p5	rps15-ycf1	5	AGCAGATCCTGTGCCTTTTT	AGTTGATTCGCCATGCTCAA	20	20	45.0	45.0	57.7	58.2	2.0	0.0	0.474236	123736	124869	1247-1271	1.000000	77.7
trnR-ACG-trnT-UGU_p1	trnR-ACG-trnT-UGU	1	AAAAGTGGTTCCTGGAACGG	ACGTTCTCCTGCCTCGATAT	20	20	50.0	50.0	58.3	58.3	28.1	0.0	0.618811	153758	154855	1293-1309	1.000000	76.6
trnR-ACG-trnT-UGU_p2	trnR-ACG-trnT-UGU	2	AAAAGTGGTTCCTGGAACGG	TACGTTCTCCTGCCTCGATA	20	20	50.0	50.0	58.3	57.7	28.1	0.0	0.649382	153758	154855	1294-1310	1.000000	76.4
trnR-ACG-trnT-UGU_p3	trnR-ACG-trnT-UGU	3	AAAAGTGGTTCCTGGAACGG	TACTACGTTCTCCTGCCTCG	20	20	50.0	55.0	58.3	58.6	28.1	0.0	0.935894	153758	154855	1297-1313	1.000000	74.1
trnR-ACG-trnT-UGU_p4	trnR-ACG-trnT-UGU	4	AAAAGTGGTTCCTGGAACGG	TCTACTACGTTCTCCTGCCT	20	20	50.0	50.0	58.3	57.2	28.1	0.0	1.115467	153758	154855	1299-1315	1.000000	72.7
trnR-ACG-trnT-UGU_p5	trnR-ACG-trnT-UGU	5	AAGTGGTTCCTGGAACGGAT	ACGTTCTCCTGCCTCGATAT	20	20	50.0	50.0	58.9	58.3	28.1	0.0	1.241549	153758	154855	1291-1307	1.000000	71.7