Hotspot

rpl16-rps3

LSC · 95637-98188 · 2552 bp

Back to Speranskia

Pi 0.121

MarkerSeek score 81.20

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 2552
variable_sites: 405
pi: 0.121
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	ATCCTTTCCCCGAACCCATA	ACGGAAAAGAAATTGCACGC	50.0	45.0	58.1	58.2	2918-2921 bp	0.666667	76.0
rpl16-rps3_p2	ATCCTTTCCCCGAACCCATA	GGAAAAGAAATTGCACGCGT	50.0	45.0	58.1	58.2	2916-2919 bp	0.666667	76.0
rpl16-rps3_p3	GACCTGGTTTAACGACAGCT	GGAAAAGAAATTGCACGCGT	50.0	45.0	57.8	58.2	2952-2955 bp	0.666667	75.5
rpl16-rps3_p4	CCGAACCCATACGTGTTTCT	ACGGAAAAGAAATTGCACGC	50.0	45.0	57.9	58.2	2912 bp	0.333333	50.8
rpl16-rps3_p5	CCGAACCCATACGTGTTTCT	GGAAAAGAAATTGCACGCGT	50.0	45.0	57.9	58.2	2910 bp	0.333333	50.8