Hotspot

clpP-psbB

LSC · 85300-86165 · 866 bp

Back to Speranskia

Pi 0.0804

MarkerSeek score 84.50

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 866
variable_sites: 77
pi: 0.0804
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CCCAGGATTTCGAAAAGGGA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.5	58.2	650-1080 bp	1.0	85.0
clpP-psbB_p2	TCCCCAGGATTTCGAAAAGG	CGATACCAAGGCAAACCCAT	50.0	50.0	57.5	58.2	652-1082 bp	1.0	85.0
clpP-psbB_p3	CCCCAGGATTTCGAAAAGGG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.5	58.2	651-1081 bp	1.0	84.8
clpP-psbB_p4	CCCAGGATTTCGAAAAGGGA	CAGCAAACGACCAGGATCAT	50.0	50.0	57.5	58.3	690-1120 bp	1.0	84.5
clpP-psbB_p5	TCCCCAGGATTTCGAAAAGG	CAGCAAACGACCAGGATCAT	50.0	50.0	57.5	58.3	692-1122 bp	1.0	84.5