Hotspot

rpl33-rps18

LSC · 80365-80901 · 537 bp

Back to Speranskia

Pi 0.0801

MarkerSeek score 81.30

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 537
variable_sites: 46
pi: 0.0801
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GCCTAGTCGATTGGAATTGAGA	TCAATTCGATCCCCCGATTG	45.5	50.0	57.9	57.7	371-2108 bp	1.0	69.0
rpl33-rps18_p2	GCCTAGTCGATTGGAATTGAGA	ATTCGATCCCCCGATTGGAT	45.5	50.0	57.9	58.6	368-2105 bp	1.0	65.9
rpl33-rps18_p3	GCCTAGTCGATTGGAATTGAG	TCAATTCGATCCCCCGATTG	47.6	50.0	56.5	57.7	371-2108 bp	1.0	65.4
rpl33-rps18_p4	GCCTAGTCGATTGGAATTGAGA	CAATTCGATCCCCCGATTGG	45.5	55.0	57.9	58.8	370-2107 bp	1.0	64.9
rpl33-rps18_p5	GCCTAGTCGATTGGAATTGAGA	AATTCGATCCCCCGATTGGA	45.5	50.0	57.9	58.9	369-2106 bp	1.0	64.1