Hotspot

psbZ-trnG-UCC

LSC · 43988-45503 · 1516 bp

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Pi 0.149
MarkerSeek score 80.80
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
1516
variable_sites
258
pi
0.149
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.90
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 TCCTTAATTCTCTCATCTCTTGA CGTCTTCTCCTTGGCAAAGA 34.8 50.0 54.3 57.8 1247-2086 bp 1.0 52.0
psbZ-trnG-UCC_p2 GGTATCCTTAATTCTCTCATCTCT CGTCTTCTCCTTGGCAAAGA 37.5 50.0 55.0 57.8 1251-2090 bp 1.0 52.0
psbZ-trnG-UCC_p3 GGTATCCTTAATTCTCTCATCTCTTG CGTCTTCTCCTTGGCAAAGA 38.5 50.0 56.9 57.8 1251-2090 bp 1.0 52.0
psbZ-trnG-UCC_p4 GGTATCCTTAATTCTCTCATCTCTTGA CGTCTTCTCCTTGGCAAAGA 37.0 50.0 58.1 57.8 1251-2090 bp 1.0 52.0
psbZ-trnG-UCC_p5 ATCCTTAATTCTCTCATCTCTTGA CGTCTTCTCCTTGGCAAAGA 33.3 50.0 54.8 57.8 1248-2087 bp 1.0 52.0