Hotspot

atpF-atpH

LSC · 16949-18634 · 1686 bp

Back to Speranskia

Pi 0.168

MarkerSeek score 78.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 1686
variable_sites: 303
pi: 0.168
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	CCGTTATTGCTGCTGGTTTG	50.0	50.0	58.0	58.0	887-2053 bp	1.0	91.5
atpF-atpH_p2	CAGTGACCCAAGGAAACGAA	CGTTATTGCTGCTGGTTTGG	50.0	50.0	58.0	58.0	886-2052 bp	1.0	91.5
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	900-2066 bp	1.0	91.4
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	AGGCCAAGCTGTAGAAGGTA	50.0	50.0	58.0	58.0	817-1983 bp	1.0	91.3
atpF-atpH_p5	CAGTGACCCAAGGAAACGAA	ATCCATTGATTTCTGCCGCT	50.0	45.0	58.0	57.9	908-2074 bp	1.0	91.2